◎ Accura Triart의 특징
1. Column의 접액부 전체를 Bioinert Coating 처리
2. 핵산과 Peptide, 단백질 등의 흡착성, 배위성 성분 분석에 최적
3. 낮은 흡착과 낮은 Carry over로 LC-MS에서 고감도 분석 실현
4. Organic Silica Hybrid의 YMC-Triart 입자를 적용해서 높은 내구성 확보
5. 다양한 관능기와 Pore size 제품의 Line-up으로 저분자부터 중·고분자까지 분석
■ Column 흡착 저감
Accura와 SUS Column을 입자의 충전 없이 LC와 연결해서 흡착력이 높은 올리고핵산의 용출을 비교했습니다. SUS Column에서는
초기 시료 주입 때부터 흡착이 크고, 연속적으로 시료를 주입해, SUS 표면에 올리고핵산이 흡착된 후에 조금씩 개선되지만,
20회를 주입해도 Accura보다 시료 용출률이 낮습니다. Accura는 처음부터 시료 흡착없이 안정적이고 양호한 감도와 회수율을 얻습니다.
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Elunet Sample | : 8 mM TEA* 200 mM HFIP**/methanol (82/18) : All PS RNA 20mer |
*triethylamine **1,1,1,3,3,3 hexafluoro-2-propanol |
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■ 올리고핵산의 저흡착·고감도 분석 실현
Triart Bio C18 Column의 Accura와 SUS 제품을 통해서 올리고핵산 분석했습니다.
Accura에서는 SUS Column보다 흡착이 억제되어 Peak 높이가 높고 고감도로 검출할 수 있습니다.
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| 1. All PS RNA 20mer 2. All PS RNA 21mer |
| Column Eluent Flow rate Temp. Detection | : Triart Bio C18 (1.9 µm, 30 nm) 50 X 2.1 mmI.D : A) 15 mM TEA 400 mM HFIP B) methanol
8-18%B (0-10 min) : 0.42 mL/min : 65°C : UV at 260 nm |
| *triethylamine **1,1,1,3,3,3 hexafluoro-2-propanol |
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■ AAV(Adeno-associated virus) 분석
Accura Triart Bio C4는 AAV capsid protein 분석에서도 우수한 분리능을 확보해서 일반적인 C4 Column에서는
분석이 어려운 VP1과 VP2를 양호하게 분리할 수 있습니다.
| | Denatured AAV2 1. VP2 : 139A-735L
2. VP1(Ac) : 2A(Ac)-735L
3. VP3(Ac) : 204A(Ac)-735L
4. VP3(Ac) variant : 212A(Ac)-735L |
| Column Eluent Flow rate Temp. Detection | : 150 X 2.1 mmI.D : A) water/difluoroacetic acid (100/0.1） B) acetonitrile/difluoroacetic acid (100/0.1）
20-32%B (0-1 min), 32-36%B (1-16 min),
36-80%B (16-20 min) : 0.2 mL/min : 80°C : UV at 280 nm |
| Courtesy of Prof. S. Uchiyama, Osaka University
This research was supported by AMED under Grant Number JP18ae0201001. |
■ Accura Triart C18 | | ■ Accura Triart C18 ExRS |
Particle size(um) | Pore size(nm) | Column size(mm) | PN. | 1.9 | 12 | 2.1 × 50 | TA12SP9-05Q1PTC | 2.1 × 100 | TA12SP9-10Q1PTC | 2.1 × 150 | TA12SP9-15Q1PTC | 3 | 12 | 2.1 × 50 | TA12S03-05Q1PTC | 2.1 × 100 | TA12S03-10Q1PTC | 2.1 × 150 | TA12S03-15Q1PTC | 4.6 × 50 | TA12S03-0546PTC | 4.6 × 100 | TA12S03-1046PTC | 4.6 × 150 | TA12S03-1546PTC | 5 | 12 | 2.1 × 50 | TA12S05-05Q1PTC | 2.1 × 100 | TA12S05-10Q1PTC | 2.1 × 150 | TA12S05-15Q1PTC | 4.6 × 50 | TA12S05-0546PTC | 4.6 × 100 | TA12S05-1046PTC | 4.6 × 150 | TA12S05-1546PTC |
| | Particle size(um) | Pore size(nm) | Column size(mm) | PN. | 1.9 | 8 | 2.1 × 50 | TAR08SP9-05Q1PTC | 2.1 × 100 | TAR08SP9-10Q1PTC | 2.1 × 150 | TAR08SP9-15Q1PTC | 3 | 8 | 2.1 × 50 | TAR08S03-05Q1PTC | 2.1 × 100 | TAR08S03-10Q1PTC | 2.1 × 150 | TAR08S03-15Q1PTC | 4.6 × 50 | TAR08S03-0546PTC | 4.6 × 100 | TAR08S03-1046PTC | 4.6 × 150 | TAR08S03-1546PTC | 5 | 8 | 2.1 × 50 | TAR08S05-05Q1PTC | 2.1 × 100 | TAR08S05-10Q1PTC | 2.1 × 150 | TAR08S05-15Q1PTC | 4.6 × 50 | TAR08S05-0546PTC | 4.6 × 100 | TAR08S05-1046PTC | 4.6 × 150 | TAR08S05-1546PTC |
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■ Accura Triart C8 | | ■ Accura Triart Bio C18 |
Particle size(um) | Pore size(nm) | Column size(mm) | PN. | 1.9 | 12 | 2.1 × 50 | TO12SP9-05Q1PTC | 2.1 × 100 | TO12SP9-10Q1PTC | 2.1 × 150 | TO12SP9-15Q1PTC | 3 | 12 | 2.1 × 50 | TO12S03-05Q1PTC | 2.1 × 100 | TO12S03-10Q1PTC | 2.1 × 150 | TO12S03-15Q1PTC | 4.6 × 50 | TO12S03-0546PTC | 4.6 × 100 | TO12S03-1046PTC | 4.6 × 150 | TO12S03-1546PTC | 5 | 12 | 2.1 × 50 | TO12S05-05Q1PTC | 2.1 × 100 | TO12S05-10Q1PTC | 2.1 × 150 | TO12S05-15Q1PTC | 4.6 × 50 | TO12S05-0546PTC | 4.6 × 100 | TO12S05-1046PTC | 4.6 × 150 | TO12S05-1546PTC |
| | Particle size(um) | Pore size(nm) | Column size(mm) | PN. | 1.9 | 30 | 2.1 × 50 | TA30SP9-05Q1PTC | 2.1 × 100 | TA30SP9-10Q1PTC | 2.1 × 150 | TA30SP9-15Q1PTC | 3 | 30 | 2.1 × 50 | TA30S03-05Q1PTC | 2.1 × 100 | TA30S03-10Q1PTC | 2.1 × 150 | TA30S03-15Q1PTC | 4.6 × 50 | TA30S03-0546PTC | 4.6 × 100 | TA30S03-1046PTC | 4.6 × 150 | TA30S03-1546PTC | 5 | 30 | 2.1 × 50 | TA30S05-05Q1PTC | 2.1 × 100 | TA30S05-10Q1PTC | 2.1 × 150 | TA30S05-15Q1PTC | 4.6 × 50 | TA30S05-0546PTC | 4.6 × 100 | TA30S05-1046PTC | 4.6 × 150 | TA30S05-1546PTC |
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■ Accura Triart Bio C4 | | ■ Accura Triart Diol-HILIC |
Particle size(um) | Pore size(nm) | Column size(mm) | PN. | 1.9 | 30 | 2.1 × 50 | TB30SP9-05Q1PTC | 2.1 × 100 | TB30SP9-10Q1PTC | 2.1 × 150 | TB30SP9-15Q1PTC | 3 | 30 | 2.1 × 50 | TB30S03-05Q1PTC | 2.1 × 100 | TB30S03-10Q1PTC | 2.1 × 150 | TB30S03-15Q1PTC | 4.6 × 50 | TB30S03-0546PTC | 4.6 × 100 | TB30S03-1046PTC | 4.6 × 150 | TB30S03-1546PTC | 5 | 30 | 2.1 × 50 | TB30S05-05Q1PTC | 2.1 × 100 | TB30S05-10Q1PTC | 2.1 × 150 | TB30S05-15Q1PTC | 4.6 × 50 | TB30S05-0546PTC | 4.6 × 100 | TB30S05-1046PTC | 4.6 × 150 | TB30S05-1546PTC |
| | Particle size(um) | Pore size(nm) | Column size(mm) | PN. | 1.9 | 12 | 2.1 × 50 | TDH12SP9-05Q1PTC | 2.1 × 100 | TDH12SP9-10Q1PTC | 2.1 × 150 | TDH12SP9-15Q1PTC | 3 | 12 | 2.1 × 50 | TDH12S03-05Q1PTC | 2.1 × 100 | TDH12S03-10Q1PTC | 2.1 × 150 | TDH12S03-15Q1PTC | 4.6 × 50 | TDH12S03-0546PTC | 4.6 × 100 | TDH12S03-1046PTC | 4.6 × 150 | TDH12S03-1546PTC | 5 | 12 | 2.1 × 50 | TDH12S05-05Q1PTC | 2.1 × 100 | TDH12S05-10Q1PTC | 2.1 × 150 | TDH12S05-15Q1PTC | 4.6 × 50 | TDH12S05-0546PTC | 4.6 × 100 | TDH12S05-1046PTC | 4.6 × 150 | TDH12S05-1546PTC |
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■ Accura Triart Phenyl | | ■ Accura Triart PFP |
Particle size(um) | Pore size(nm) | Column size(mm) | PN. | 1.9 | 12 | 2.1 × 50 | TPH12SP9-05Q1PTC | 2.1 × 100 | TPH12SP9-10Q1PTC | 2.1 × 150 | TPH12SP9-15Q1PTC | 3 | 12 | 2.1 × 50 | TPH12S03-05Q1PTC | 2.1 × 100 | TPH12S03-10Q1PTC | 2.1 × 150 | TPH12S03-15Q1PTC | 4.6 × 50 | TPH12S03-0546PTC | 4.6 × 100 | TPH12S03-1046PTC | 4.6 × 150 | TPH12S03-1546PTC | 5 | 12 | 2.1 × 50 | TPH12S05-05Q1PTC | 2.1 × 100 | TPH12S05-10Q1PTC | 2.1 × 150 | TPH12S05-15Q1PTC | 4.6 × 50 | TPH12S05-0546PTC | 4.6 × 100 | TPH12S05-1046PTC | 4.6 × 150 | TPH12S05-1546PTC |
| | Particle size(um) | Pore size(nm) | Column size(mm) | PN. | 1.9 | 12 | 2.1 × 50 | TPF12SP9-05Q1PTC | 2.1 × 100 | TPF12SP9-10Q1PTC | 2.1 × 150 | TPF12SP9-15Q1PTC | 3 | 12 | 2.1 × 50 | TPF12S03-05Q1PTC | 2.1 × 100 | TPF12S03-10Q1PTC | 2.1 × 150 | TPF12S03-15Q1PTC | 4.6 × 50 | TPF12S03-0546PTC | 4.6 × 100 | TPF12S03-1046PTC | 4.6 × 150 | TPF12S03-1546PTC | 5 | 12 | 2.1 × 50 | TPF12S05-05Q1PTC | 2.1 × 100 | TPF12S05-10Q1PTC | 2.1 × 150 | TPF12S05-15Q1PTC | 4.6 × 50 | TPF12S05-0546PTC | 4.6 × 100 | TPF12S05-1046PTC | 4.6 × 150 | TPF12S05-1546PTC |
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